Bureau of Reclamation: High-Throughput Markers Development for Rio Grande Silvery Minnow

The Bureau of Reclamation is offering a grant to develop Single Nucleotide Polymorphisms (SNP) panel through Restriction Site-Associated DNA Sequencing for monitoring genetic diversity of the endangered Rio Grande silvery minnow. The project aims to create markers for genetic metrics evaluation, including genetic diversity, effective population size, and population structure. Additionally, sex-specific markers will be developed for determining gender. The grant requires the submission of study plans, updates, and reports for review. The principal place of performance for this grant will be at the University of New Mexico.

Opportunity ID: 308769

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General Information

Document Type:	Grants Notice
Funding Opportunity Number:	BOR-UC-18-N019
Funding Opportunity Title:	Development of High-Throughput Markers for Rio Grande Silvery Minnow
Opportunity Category:	Discretionary
Opportunity Category Explanation:	–
Funding Instrument Type:	Other
Category of Funding Activity:	Natural Resources
Category Explanation:	–
Expected Number of Awards:	1
Assistance Listings:	15.517 — Fish and Wildlife Coordination Act
Cost Sharing or Matching Requirement:	No
Version:	Synopsis 1
Posted Date:	Sep 05, 2018
Last Updated Date:	–
Original Closing Date for Applications:	Sep 19, 2018
Current Closing Date for Applications:	Sep 19, 2018
Archive Date:	Sep 30, 2018
Estimated Total Program Funding:	$346,728
Award Ceiling:	$346,728
Award Floor:	$1

Eligibility

Eligible Applicants:	Public and State controlled institutions of higher education
Additional Information on Eligibility:	–

Additional Information

Agency Name:	Bureau of Reclamation
Description:	Monitoring of the genetics of the endangered Rio Grande silvery minnow (RGSM) has been ongoing since 1999, using nine microsatellite loci and the mitochondrial ND4 gene. From these nine microsatellite loci and ND4 gene, genetic diversity metrics are calculated and recommendations for genetic management decisions are made. The ongoing silvery minnow genetic monitoring program is a requirement in the 2016 Final Biological and Conference Opinion for Bureau of Reclamation, Bureau of Indian Affairs, and Non-Federal Water Management and Maintenance Activities on the Middle Rio Grande in New Mexico (BO). Development of high throughput markers will help meet the BO requirement. This effort is also in response to the recommendations of the Peer Review Panel that examined the RGSM genetic monitoring program in 2016. The principal place of performance will be the University of New Mexico. The purpose of this project is the development of a Single Nucleotide Polymorphisms (SNP) panel via Restriction Site-Associated DNA Sequencing that will allow for a thorough evaluation of various genetic metrics within silvery minnow. The project objectives are to: 1. Develop a SNP panel that provides sufficient loci to monitor genetic diversity (spatiotemporal), effective population size, population structure, and relatedness within or among in situ and ex situ populations. 2. Develop sex-specific markers that will allow genetic determination of sex. 3. Provide plans, updates, and reports for review and approval, including a draft study plan, oral presentations, and draft/final reports.
Link to Additional Information:	–
Grantor Contact Information:	If you have difficulty accessing the full announcement electronically, please contact: Ossie Taylor Grants Management Specialist Phone 801-524-3806 Email:otaylor@usbr.gov

Version History

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