The USGS, through the Southern Appalachian CESU, is offering this grant to a University partner for collaborative research on water quality. This grant is for analyzing and interpreting existing environmental data and conducting advanced lab analysis of water samples. The work involves fecal source tracking using real-time PCR to amplify Bacteroides sp. (AllBac, HuBac, BoBac) and specific human pathogen assays (Salmonella, E. coli markers). The successful partner will calculate molecular copy counts and ratios to estimate fecal loads in streams and identify fecal sources (human or bovine). Additionally, the grantee will actively participate in data synthesis and publication. Applicants must demonstrate intimate familiarity with the study data, possess in-house real-time PCR capabilities, and have a proven record of successful bacterial source tracking applications reflected in peer-reviewed publications.
Opportunity ID: 52135
General Information
| Document Type: | Grants Notice |
| Funding Opportunity Number: | 10HQPA0040 |
| Funding Opportunity Title: | Cooperative Ecosystem Studies Unit, Southern Appalacian CESU |
| Opportunity Category: | Discretionary |
| Opportunity Category Explanation: | – |
| Funding Instrument Type: | Cooperative Agreement |
| Category of Funding Activity: | Science and Technology and other Research and Development |
| Category Explanation: | – |
| Expected Number of Awards: | 1 |
| Assistance Listings: | 15.808 — U.S. Geological Survey_ Research and Data Collection |
| Cost Sharing or Matching Requirement: | No |
| Version: | Synopsis 1 |
| Posted Date: | Feb 25, 2010 |
| Last Updated Date: | – |
| Original Closing Date for Applications: | Mar 09, 2010 |
| Current Closing Date for Applications: | Mar 09, 2010 |
| Archive Date: | Apr 08, 2010 |
| Estimated Total Program Funding: | $17,000 |
| Award Ceiling: | $0 |
| Award Floor: | $0 |
Eligibility
| Eligible Applicants: | Others (see text field entitled “Additional Information on Eligibility” for clarification) |
| Additional Information on Eligibility: | This financial assistance opportunity is being issued under a Cooperative Ecosystems Studies Unit (CESU) Program. CESU’s are partnerships that provide research, technical assistance, and education. Eligible recipients must be a participating partner of the Southern Appalachian Cooperative Ecosystem Studies Unit (CESU) Program. |
Additional Information
| Agency Name: | Geological Survey |
| Description: | The successful University partner will collaborate with USGS scientists in analyzing and interpreting previously collected data as well as additional lab analysis of water samples provided by the USGS. The additional analysis is a fecal source tracking assay based on real-time polymerase chain reaction amplification of a region of the 16S rRNA gene of Bacteroides sp and a limited number of human pathogen assays. The human pathogen assays to be performed will be negotiated on a case-by-case basis and should include at least three assays (Salmonella and two E. coli pathogen markers). Each Bacteroides analysis will consist of three assays (AllBac, HuBac, and BoBac). The University partner should have the capability to use the resulting molecular data to calculate counts of Bacteroides molecular copies (human, bovine, and total) and ratios of said counts among each type. Ratios between the molecular copies of E. coli and total molecular Bacteroides copies should also be calculated. The ability to use the calculated results to estimate fecal loads in the studied streams is also required. The interpretation of the results should include the implication of the calculated ratios as to the source of fecal material (human or bovine) at each of the study sites. In addition, the successful Universitry partner will participate actively in analysis, synthesis, and publication of the entire data sets collected for this studsy. The University providing this support must demonstrate (1) intimate familiarity with the data collected to date for this study, (2) in-house real-time PCR capabilities, and (3) a record of successful application of real-time PCR assays to bacteria source tracking in reflected in peer-reviewed publications on the topic. |
| Link to Additional Information: | – |
| Grantor Contact Information: | If you have difficulty accessing the full announcement electronically, please contact:
FAITH GRAVES
Contract Specialist Phone 703-648-7356 Email:fgraves@usgs.gov |
Version History
| Version | Modification Description | Updated Date |
|---|---|---|
Related Documents
Folder 52135 Full Announcement-1 -> announcement.pdf
Packages
| Agency Contact Information: | FAITH GRAVES Contract Specialist Phone 703-648-7356 Email: fgraves@usgs.gov |
| Who Can Apply: | Organization Applicants |
| Assistance Listing Number | Competition ID | Competition Title | Opportunity Package ID | Opening Date | Closing Date | Actions |
|---|---|---|---|---|---|---|
| 15.808 | 10HQPA0040 | Cooperative Ecosystem Studies Unit, Southern Appalachian CESU | PKG00016682 | Feb 25, 2010 | Mar 09, 2010 | View |
Package 1
Mandatory forms
52135 SF424-2.0.pdf
52135 Project-1.1.pdf
52135 SF424B-1.1.pdf
52135 SF424A-1.0.pdf
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